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Michael Hooker Microscopy Facility (MHMF.ORG)

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Using the LeicaSP2-AOBS Confocal Microscope
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1.  Powering Up
        1.1  Assess the status of the system
        1.2  Turn on mercury arc lamp, microscope stand and scanner
        1.3  Turn on the required laser(s)
        1.4  Log into WindowsXP
2.  Viewing the Sample (microscope only)
        2.1  Setting the stage (general information for fluorescence or transmitted light viewing)
        2.2  Transmitted light (specific setup instructions)
        2.3  DIC/Nomarski (if required)
        2.4  Fluorescence
3. Starting the Confocal Scanning Program
4. Scanning the sample
        Image Overlay
5. Saving Images (brief description--this section is incomplete at this time)
6. Shutting Down - All USERS MUST FOLLOW SHUT DOWN PROCEDURES CAREFULLY
7.
Confocal Scanning Tasks (this section is incomplete at this time)
        - X-Z Scanning (brief description)
        - Z-series (brief description)
8. Image Processing (this section is incomplete at this time)
        - Adding Scale Bars to your image (requires the full Leica LCS software)


** If you have any questions, please contact a facility director **


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Back to Hooker Microscopy Facility main page

Michael Hooker Microscopy Facility (MHMF.ORG)

Go back

Using the LeicaSP2-AOBS Confocal Microscope

** If you have ANY questions, please contact a facility director **

1. Powering Up - assuming entire system is off

        1.1  Assess status of the system

        1.2  Turn on mercury arc lamp, microscope stand and scanner

        1.3  Turn on the required laser(s)
                      **Only turn on laser which are required for the dyes in your samples**
                      Properly power down any laser which is not required (see section 8 below)

    1.4  Log on to WindowsXP

3. Starting the Confocal Scanning Program

2.  Viewing the Sample

2.1  Setting the stage (general information for fluorescence or transmitted light viewing)

        For fluorescence only go to section 2.4 (skip sections 2.2 & 2.3)

        2.2  Transmitted light (specific setup instructions)

        2.2a For Imaging transmitted light, set up Kohler illumination
               
Kohler illumination is not important for fluorescence or confocal imaging. 
                However, if simultaneous confocal and transmitted scanning are done, Kohler illumination should be set up

        2.3  DIC/Nomarski - (if required)

        2.4  Fluorescence

4. Scanning the sample

 

5. Saving Images (brief description below)

Return to looking through the microscope by pressing the MicCtrl button and choosing Visual


6. Shutting Down - ALL users MUST follow these shut down procedures carefully


7. Confocal Scanning Tasks

8. Image Processing


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Copyright 2001-2018  Dr. M. Chua, School of Medicine, University of North Carolina, Chapel Hill, NC 27599
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Last Updated: 2015-12-28